CytoQuest™ CR

Advanced Microfluidic System for Positive Enrichment and Retrieval of CTCs, CSCs, and EVTs

Abnova's CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs).Three major subtypes of CRCs in translational research and clinical studies are circulating tumor cells (CTCs), circulating cancer stem cells (CSCs), and circulating extravillous trophoblasts (EVTs). A challenge for market adoption of CRCs is the efficient and reproducible identification, single cell isolation, and retrieval of highly pure and viable CRCs, with their applications supported by a wide repertoire of standardized, GMP grade bioreagents.

CytoQuest™ CR technology utilizes SCx™ spiral chamber, HBx™ micromixer, antibody immobilized nanoarray, and TCx™ thermal control to enable a multitude of CRC functions. SCx™ spiral chamber is equipped with a non-sticky coil and a self-contained microvibrator for unimpeded delivery of pretreated blood sample into the nanoarray. HBx™ micromixer provides a herringbone conduit for cell mixing. CytoChipNano is a streptavidin nanoarray which captures the CRCs for cell enumeration and single cell isolation by laser microdissection or micromanipulation. CytoChipNano CR is a specialized streptavidin nanoarray with thermo-sensitive coating which captures and releases the CRCs via a TCx™ thermal control of alternating temperatures.

Monoclonal antibodies targeting specific surface biomarkers of circulating rare cells can be flexibly interchanged to accommodate the biomarker switch in CRC progression. This results in highly pure and viable CRCs for downstream protein characterizations, gene analyses, and cell assays. Cell enumeration is currently the only FDA approved application as a prognostic marker in breast, prostate, and colorectal cancers. In contrast, effective single cell isolation and retrieval of CRCs opens up new scope of applications in the diagnostic and pharmaceutical industry.

 

 

Subtypes of CRCs

 

  • Circulating Tumor Cells (CTCs)
  • Cancer Stem Cells (CSCs)
  • Extravillous Trophoblasts (EVTs)


Applications of CTCs

 

Protein characterizations

  • Immunofluorescence
  • Proximity ligation assay (PLA)

Gene analyses

  • Fluorescent In Situ Hybridization (FISH)
  • Polymerase Chain Reaction (PCR)
  • Comparative Genomic Hybridization (CGH)
  • Next Generation Sequencing (NGS)

Cell assays

  • Primary cell line construction
  • Compound screening


Advantages of CytoQuest™ CR Platform

 

Integrated system

  • Bottle reservoir
  • Fluidic handler
  • SCx™ spiral chamber
  • TBx™ tension balancer
  • Coupler
  • HBx™ micromixer
  • Chip receptor
  • TCx™ thermal chuck
  • Touch user interface

Antibody-Based, Cell Capture

  • Cell enumeration (CytoChipNano)
  • Single cell isolation (CytoChipNano)
  • Thermal cell release (CytoChipNano CR)

 

CytoQuest™ CR

 

 

 




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